Masters Theses

Abstract

"Pigmented bacteria have been of interest to the microbiologist for several years. The pigmented organisms in sewage have been known to gain predominance, but no investigations have been made to determine whether the pigment assists the organism in gaining predominance. During this study a number of bacteria which produced a water soluble pigment were isolated from sewage. These bacteria were grown in shaken flasks using tryptone Glucose extract broth as a medium. Growth studies of the bacteria in pure and mixed culture were performed to determine if the pigment could affect another bacteria's growth. Studies were also performed using the medium supernatant and filtrate following the growth of one species as a medium supplement for growth of another species of bacteria. Growth rates were measured directly by colony counts and indirectly by optical density at 540 mµ. Substrate concentration was determined by the COD test and by the total orgainic carbon test using a carbonaceous analyzer. Tryptone glucose extract agar was used for colony enumeration. It was found that the pigmented organisms used in this study increased the pH of the mixed liquor from 7.0 to 7.7-8.0 and that the pigment in small quantities may tend to stimulate growth whereas larger quantities may become inhibitory to the organisms studied"--Abstract, page ii.

Advisor(s)

Modesitt, Donald E.

Committee Member(s)

Jennett, J. Charles
Hufham, James B.

Department(s)

Civil, Architectural and Environmental Engineering

Degree Name

M.S. in Civil Engineering

Sponsor(s)

United States. Environmental Protection Agency

Publisher

University of Missouri--Rolla

Publication Date

1971

Pagination

vii, 58 pages

Rights

© 1971 Leslie Alexander Lakie, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Library of Congress Subject Headings

Sewage -- Analysis
Bacteria -- Analysis
Microbial pigments
Biochemical oxygen demand

Thesis Number

T 2641

Print OCLC #

6039118

Electronic OCLC #

876717136

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