Keywords and Phrases
"Luminescent semiconductor quantum dots (QDs) have recently been used for delivering and monitoring biomolecules, such as drugs and proteins. However, QDs alone have a very low efficiency of transport across the plasma membrane. In order to increase the efficiency of QD delivery, synthetic nona-arginine (sR9) was used, a cell penetrating peptide, to facilitate uptake. Data demonstrated that sR9 could significantly increase the cellular uptake of QDs by noncovalent binding between QDs and sR9. Furthermore, the mechanisms of QD/sR9 cellular internalization were investigated. Low temperature and metabolic inhibitors markedly inhibited the uptake of QD/sR9, indicating that internalization is an energy-dependent process. Several pathway inhibitors and the RNAi technique were used to analyze the mechanism of uptake in live cell imaging studies. siRNA knockdown demonstrated that clathrin-, and caveolin-dependent endocytosis were not involved in QD/sR9 internalization. The conclusion is that the major routes of cellular uptake involve macropinocytosis and lipid-raft dependent process"--Abstract, page iii.
M.S. in Applied and Environmental Biology
Missouri University of Science and Technology
ix, 45 pages
© 2009 Yi Xu, All rights reserved.
Thesis - Open Access
Library of Congress Subject Headings
Biological transport -- Research
Print OCLC #
Electronic OCLC #
Link to Catalog Recordhttp://laurel.lso.missouri.edu/record=b7463688~S5
Xu, Yi, "Nona-arginine peptides facilitate cellular entry of semiconductor nanocrystals: mechanisms of uptake" (2009). Masters Theses. 4735.