"The Hens Egg Test on the Chorionallantoic Membrane (HET-CAM) is an ocular irritation test proposed to replace tradition ocular investigations such as the Draize eye test that has been criticized because of its use of animals. In this study, the ocular irritation potential of seawater and saltwater sources dosed with different disinfectants has been evaluated using the HET-CAM. The seawater and salt water tested was taken from the main show pools from three aquatic theme parks (San Diego, San Antonio, and Orlando). The three disinfectants considered were sodium hypochlorite (NaOCl), calcium hypochlorite (Ca(OCl)₂), and ozone (O₃).
Three different HET-CAM methods were performed. Egg preparation using Method One proved to be very difficult, and very few signs of irritation were observed. Egg preparation using Method Two proved much easier, but the new setup helped induce the irritation observed. Finally, egg preparation using Method Three was similar to Method Two, but alleviated the affect of irritation induced by outside factors.
The development of a working method left little time to obtain pertinent results. Nevertheless, as suggested by Erdinger et al., a synergistic effect was observed and may contribute to the ocular irritation induced. Also, the ocular irritation potential of Ca(OCl)₂ appeared to be mildly less offensive than CaOCI.
Ultimately, the results at hand do help to serve as a guideline for anyone who may wish to pursue this project/method further"--Abstract, page iii.
Adams, C. D. (Craig D.)
Burken, Joel G. (Joel Gerard)
Civil, Architectural and Environmental Engineering
M.S. in Environmental Engineering
Busch Entertainment Corp.
Missouri University of Science and Technology
x, 52 pages
© 2009 Alexander Clovis Winters, All rights reserved.
Thesis - Restricted Access
Library of Congress Subject Headings
Eye -- Diseases -- Diagnosis
Seawater -- Testing
Calcium hypochlorite -- Testing
Sodium hypochlorite -- Testing
Print OCLC #
Electronic OCLC #
Link to Catalog RecordElectronic access to the full-text of this document is restricted to Missouri S&T users. Otherwise, request this publication directly from Missouri S&T Library or contact your local library.http://laurel.lso.missouri.edu:80/record=b10251202~S5
Winters, Alexander Clovis, "HET-CAM for the identification of ocular irritancy in seawater/salt water sources" (2009). Masters Theses. 4516.