Masters Theses

Abstract

"The engineered regeneration of bone is a significant challenge being undertaken to treat conditions such as traumatic casualties, bone cancer, osteoporosis, etc. Advances in hard tissue regrowth may potentially lead to significantly improved lives for millions of people. Bioactive glasses as potential materials in the fabrication of scaffolds for hard tissue regeneration have been studied recently. Bone regrowth requires maintenance of optimal levels of oxygen, glucose, phosphate, calcium, and pH. Current work at Missouri S & T's Center for Bone and Tissue Repair and Restoration focuses on developing optical biosensors to monitor: conversion of bioactive glass to hydroxyapatite, ease of nutrient transport through the scaffold, diffusion of bioconversion byproducts from the wound site, and general health of the growing cells. Feedback from these sensors aids in material design, allowing researchers to understand how desired levels of analyte molecules are maintained in the complex process of tissue ingrowth.

Our work focuses on development of an oxygen biosensor element/platform, employing Pt(II) meso-tetra(pentafluorophenyl)porphine complex immobilized in a poly(dimethyl-siloxane) membrane, to monitor bioactive conversion process in simulated physiological saline solution environment. Phosphorescence of the dye quenches with increase in surrounding oxygen levels, and this difference leads to an image of oxygen gradients which develop in the matrix. A CCD camera and processing software is used to colorimetrically quantify oxygen levels at the microscale. Image processing is done in the RGB (red, green, blue - native to the camera) color space, giving detailed information about analyte concentration throughout the scaffolds and allowing for real time, in situ monitoring of cellular ingrowth"--Abstract, page iv.

Advisor(s)

Xing, Yangchuan
Henthorn, David

Committee Member(s)

Kim, Chang-Soo
Day, D. E.

Department(s)

Chemical and Biochemical Engineering

Degree Name

M.S. in Chemical Engineering

Publisher

Missouri University of Science and Technology

Publication Date

Spring 2011

Journal article titles appearing in thesis/dissertation

  • Simple oxygen imaging method based on color camera and an oxygen sensor film
  • Continuous O₂ monitoring around degrading bioactive glass scaffold using an optical biosensor element

Pagination

xi, 95 pages

Note about bibliography

Includes bibliographical references.

Rights

© 2011 Prajakta Bhagwat, All rights reserved.

Document Type

Thesis - Restricted Access

File Type

text

Language

English

Library of Congress Subject Headings

Biomedical materials -- Testing
Biosensors
Bone regeneration
Glass in medicine
Tissue scaffolds

Thesis Number

T 9803

Print OCLC #

790619361

Electronic OCLC #

908570426

Link to Catalog Record

Electronic access to the full-text of this document is restricted to Missouri S&T users. Otherwise, request this publication directly from Missouri S&T Library or contact your local library.

http://laurel.lso.missouri.edu/record=b8542899~S5

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