Purification of human antibody from transgenic corn using aqueous two-phase systems
Keywords and Phrases
"Human antibodies that are very important for the immune system can be produced in transgenic corn. However, their expression is low, less than 4% purity. The consequence of this low expression is an increase in the down-stream processing. Therefore, it is necessary to develop novel and economical purification processes to fully benefit from plant host systems. A recombinant human antibody expressed in corn was separated from its impurities using aqueous two-phase extraction. PEG/potassium phosphate, PEG/sodium citrate, and PEG/ammonium phosphate aqueous two-phase systems were used. The effect of pH, temperature, molecular weight of PEG, total concentration of the phase-forming species, and low concentration of NaCl were investigated to determine optimum separation conditions"--Abstract, leaf iii.
Reed, X. B., Jr.
Ludlow, Douglas K.
Chemical and Biochemical Engineering
Ph. D. in Chemical Engineering
Monsanto Protein Tech
University of Missouri--Rolla
ix, 89 leaves
© 2006 Jinwook Lee, All rights reserved.
Dissertation - Citation
Library of Congress Subject Headings
Polyethylene glycol -- Biotechnology
Print OCLC #
Link to Catalog Record
Full-text not available: Request this publication directly from Missouri S&T Library or contact your local library.http://laurel.lso.missouri.edu/record=b5794141~S5
Lee, Jinwook, "Purification of human antibody from transgenic corn using aqueous two-phase systems" (2006). Doctoral Dissertations. 1663.
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