Title

Cell-Sensitive Microscopy Imaging for Cell Image Segmentation

Abstract

We propose a novel cell segmentation approach by estimating a cell-sensitive camera response function based on variously exposed phase contrast microscopy images on the same cell dish. Using the cell-sensitive microscopy imaging, cells' original irradiance signals are restored from all exposures and the irradiance signals on non-cell background regions are restored as a uniform constant (i.e., the imaging system is sensitive to cells only but insensitive to non-cell background). Cell segmentation is then performed on the restored irradiance signals by simple thresholding. The experimental results validate that high quality cell segmentation can be achieved by our approach.

Department(s)

Computer Science

Keywords and Phrases

Cells; Image Segmentation; Restoration; Cells; Cytology; Medical Computing; Medical Imaging; Restoration; Background Region; Camera Response Functions; Cell Image Segmentation; Cell Segmentation; High Quality; Microscopy Imaging; Phase-Contrast Microscopy Images; Thresholding; Cytology; Image Segmentation

International Standard Book Number (ISBN)

9783319104034

International Standard Serial Number (ISSN)

0302-9743

Document Type

Article - Conference proceedings

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2014 Springer Verlag, All rights reserved.

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