Fabrication of Culture-Based Biochips for Detecting Microorganisms in Environmental Samples
In this paper, we describe a culture-based biochip for detecting microorganisms in environmental samples in an improved, timesaving, and cost-efficient manner. The biochips use the paraffinophillic nature of microorganisms to rapidly detect and differentiate them in sewage samples collected from wastewater treatment plants. A new method of depositing paraffin was developed to fabricate the biochips. The developed technique uses butanol solvent to keep paraffin liquid during spin-coating and thick photoresist for patterning. Using this approach, paraffin layers of 5-70 µm have been successfully fabricated and patterned. These biochips were used to demonstrate rapid, 5-min detection of microorganisms responsible for reactor foaming in wastewater treatment.
H. B. Eluru et al., "Fabrication of Culture-Based Biochips for Detecting Microorganisms in Environmental Samples," Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology (2002, Houston, TX), vol. 2, pp. 1688-1689, Institute of Electrical and Electronics Engineers (IEEE), Oct 2002.
The definitive version is available at http://dx.doi.org/10.1109/IEMBS.2002.1106603
IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) (2002: Oct. 23-26, Houston, TX)
Civil, Architectural and Environmental Engineering
Keywords and Phrases
Activated sludge process; Biotechnology; Cell culture; Foams; Molecular biology; Paraffins; Photoresists; Sewage analysis; Spin coating; Biochips; Mycobacteria; Nocardiafoam; Paraffinophilic nature; Microorganisms
International Standard Serial Number (ISSN)
Article - Conference proceedings
© 2002 Institute of Electrical and Electronics Engineers Inc. (IEEE) , All rights reserved.