Developing Culture-Based Biochips for Detecting Microorganisms in Wastewater Treatment
In this paper, we describe a culture-based biochip for detecting microorganisms in environmental samples in an improved, timesaving, and cost-efficient manner. The biochips use the paraffinophilic nature of microorganisms to rapidly detect and differentiate them in sewage samples collected from wastewater treatment plants. A new method of depositing paraffin was developed in order to fabricate such biochips. The developed technique uses butanol solvent to keep paraffin liquid during deposition by spin-coating and thick photoresist for patterning. Using this approach, 18 mm glass biochips with paraffin layers of 5-70 µm have been successfully fabricated. These biochips were used to demonstrate rapid, 5-min detection of microorganisms responsible for reactor foaming in wastewater treatment.
H. B. Eluru et al., "Developing Culture-Based Biochips for Detecting Microorganisms in Wastewater Treatment," Proceedings of the 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology (2002, Madison, WI), pp. 152-155, Institute of Electrical and Electronics Engineers Inc. (IEEE), May 2002.
The definitive version is available at http://dx.doi.org/10.1109/MMB.2002.1002303
2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology (2002: May 2-4, Madison, WI)
Civil, Architectural and Environmental Engineering
Keywords and Phrases
Bioassay; Biochips; Microarrays; Microorganisms; Photoresists; Plants (botany); Reclamation; Sewage; Sewage treatment plants; Cost-efficient; Environmental sample; Glass biochips; mycobacteria; Thick photoresists; Wastewater treatment plants; Wastewater treatment; mycobacteria; paraffin
International Standard Book Number (ISBN)
Article - Conference proceedings
© 2002 Institute of Electrical and Electronics Engineers Inc. (IEEE) , All rights reserved.