Title

Culture-Based Biochip for Environmental Monitoring

Abstract

In this paper, we describe the development of a culture-based biochip for detecting mycobacteria in environmental samples. The biochips use the paraffinophilic nature of mycobacteria to rapidly detect and differentiate them from non-target microorganisms. New methods of depositing and patterning paraffin were developed to fabricate prototype biochips. Biochips were experimentally tested to demonstrate rapid detection of mycobacteria in environmental samples collected from a municipal sewage treatment plant. Our successful demonstration of the culture-based biochip technology presents an alternative approach for developing new technology to track microorganisms in complex environmental samples.

Meeting Name

Microfluidics, BioMEMS, and Medical Microsystems II (2004: Jan. 26-27, San Jose, CA)

Department(s)

Civil, Architectural and Environmental Engineering

Sponsor(s)

SPIE

Keywords and Phrases

Biochips; Environmental monitoring; Myobacteria; Paraffin patterning; Adhesives; Bacteria; Biomass; Cell culture; Curing; Environmental engineering; Fluorescence; Health care; Potable water; RNA; Sorption; Spin coating; Microprocessor chips

International Standard Serial Number (ISSN)

0277786X

Document Type

Article - Conference proceedings

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2004 SPIE, All rights reserved.


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