Comparison of Sterculic Oil Induced Changes in the Gut Microbiota of Lean and Obese Mice
Recent obesity research has reported differences in intestinal microbiota between obese ob/ob mice and lean wild type mice suggesting a potential link between bacterial populations and metabolic health. Sterculic oil (SO) is a natural inhibitor of stearoyl-CoA desaturase-1, a potential therapeutic target for the treatment/attenuation of obesity and insulin resistance.The aim of this study was to investigate the response of the obese and lean gut microbiota to a SO supplemented diet. Five-week old male lean (WT) and obese (ob/ob) mice were fed either a control AIN-93G diet or an AIN-93G diet containing 0.5% SO for 9 weeks Barcoded pyrosequencing of the V4-V5 region of the 16S rRNA gene was employed to characterize cecal microbiota. Intestinal microbiota from WT mice fed a control diet were more diverse, although there were no significant differences in the proportions of major phyla from WT and ob/ob mice. Irrespective of genotype, SO was negatively correlated with the relative abundances of Actinobacteria, Bacilli and Erysipelotrichi. This shift was not associated with a change in body weight, however a statistically significant improvement in glucose tolerance and insulin sensitivity was observed in the ob/ob mice fed SO. In our study, diet had a greater impact on gut microbiota as compared to genotype and these changes were associated with an improved metabolic health.
S. Ghosh et al., "Comparison of Sterculic Oil Induced Changes in the Gut Microbiota of Lean and Obese Mice," FASB Journal, vol. 26, no. 1, pp. 830.2, Federation of American Society of Experimental Biology (FASEB), Apr 2012.
Experimental Biology 2012 (2012: Apr. 21-25, San Diego, CA)
Civil, Architectural and Environmental Engineering
Missouri University of Science and Technology
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