Detection of Cations Separated by Thin-Layer Chromatography by Fluorescence Quenching of Ethidium Bromide
Cations such as Cu2+, Cr3+ and Hg+ can be separated on silica gel thin layer Chromatographic plates. The developing solution contains 0.02 M EDTA at pH 2.5. For visualization, the plate was pretreated with ethidium bromide, a strong fluorescer. Quenching of the red fluorescence can be observed visually even down to the 0.1 nmole range per spot.
Y. Ma and E. S. Yeung, "Detection of Cations Separated by Thin-Layer Chromatography by Fluorescence Quenching of Ethidium Bromide," Mikrochimica Acta, vol. 96, no. 1-6, pp. 327-332, Springer Verlag, Jan 1988.
The definitive version is available at http://dx.doi.org/10.1007/BF01236117
Keywords and Phrases
Cations; Chromatography; Fluorescence; Quenching
International Standard Serial Number (ISSN)
Article - Journal
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