Title

Detection of Cations Separated by Thin-Layer Chromatography by Fluorescence Quenching of Ethidium Bromide

Abstract

Cations such as Cu2+, Cr3+ and Hg+ can be separated on silica gel thin layer Chromatographic plates. The developing solution contains 0.02 M EDTA at pH 2.5. For visualization, the plate was pretreated with ethidium bromide, a strong fluorescer. Quenching of the red fluorescence can be observed visually even down to the 0.1 nmole range per spot.

Department(s)

Chemistry

Keywords and Phrases

Cations; Chromatography; Fluorescence; Quenching

International Standard Serial Number (ISSN)

00263672

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1988 Springer Verlag, All rights reserved.


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