Detection of Cations Separated by Thin-Layer Chromatography by Fluorescence Quenching of Ethidium Bromide

Author

Yinfa Ma, Missouri University of Science and TechnologyFollow
Edward S. Yeung

Abstract

Cations such as Cu²⁺, Cr³⁺ and Hg⁺ can be separated on silica gel thin layer Chromatographic plates. The developing solution contains 0.02 M EDTA at pH 2.5. For visualization, the plate was pretreated with ethidium bromide, a strong fluorescer. Quenching of the red fluorescence can be observed visually even down to the 0.1 nmole range per spot.

Recommended Citation

Y. Ma and E. S. Yeung, "Detection of Cations Separated by Thin-Layer Chromatography by Fluorescence Quenching of Ethidium Bromide," Microchimica Acta, vol. 96, no. 1-6, pp. 327 - 332, Springer Verlag, Jan 1988.

The definitive version is available at https://doi.org/10.1007/BF01236117

Department(s)

Chemistry

Keywords and Phrases

Cations; Chromatography; Fluorescence; Quenching

International Standard Serial Number (ISSN)

0026-3672

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1988 Springer Verlag, All rights reserved.

Publication Date

01 Jan 1988