Isolation and Quantification of Ergovaline from Festuca Arundinacea (Tall Fescue) Infected with the Fungus Acremonium Coenophialum by High-Performance Capillary Electrophoresis
We developed an easy and sensitive high-performance capillary zone electrophoresis method for the determination of ergovaline in the seeds of Festuca arundinacea (tall fescue) infected with the endophytic, ascomycetous fungus Acremonium coenophialum. The seeds were extracted with chloroform, and the chloroform extract was filtered and evaporated with a Rotavapor. The concentrated extract was passed through a SM2 Bio-Beads and the alkaloids on the Bio-Beads were redissolved by methanol. A 60 cm x 75 µm I.D. fused-silica capillary was used for separation by capillary electrophoresis. A UV detector was used for detection, which was set at 250 nm. A 0.1 M sodium dihydrogenphosphate in 50% (v/v) methanol, pH 3.5, was used as a buffer. With this method, the ergovaline at low µg per kg seeds can be detected and quantified.
Y. Ma et al., "Isolation and Quantification of Ergovaline from Festuca Arundinacea (Tall Fescue) Infected with the Fungus Acremonium Coenophialum by High-Performance Capillary Electrophoresis," Journal of Chromatography, vol. 652, no. 2, pp. 535-538, Elsevier, Oct 1993.
The definitive version is available at http://dx.doi.org/10.1016/0021-9673(93)83275-W
Keywords and Phrases
Alkaloid; Ergovaline; Unclassified Drug; Capillary Electrophoresis; Mycosis; Nonhuman; Plant Seed; Priority Journal; Zone Electrophoresis
International Standard Serial Number (ISSN)
Article - Journal
© 1993 Elsevier, All rights reserved.