Determination of Total Iron Binding Capacity of Serum by Capillary Electrophoresis
A capillary electrophoresis (CE) technique for determining total iron binding capacity (TIBC) of serum has been developed. The optimum serum pretreatment involves the following major steps: at first, saturate serum transferrin with Fe+3; then, dissociate them completely after removing excess unbound Fe. Finally, complex the released iron with phenanthroline, a chromophore, to make suitable for the CE analysis. Ammonium acetate (pH = 5.0) was used as CE background electrolyte solution. In this system, a good linear correlation coefficient was maintained over the range 0.5~10 µM (r = 0.9979, n=12). Seven adult serum samples were studied and the TIBC parameters measured. In the present system, 10~30 µL serum is sufficient for determination. The study shows that the CE technique described is a powerful method for rapid, efficient, sensitive and reliable analysis and hence particularly suitable for clinical application.
Y. Ding et al., "Determination of Total Iron Binding Capacity of Serum by Capillary Electrophoresis," Chromatographia, vol. 49, no. 1-2, pp. 71-74, Springer Verlag, Jan 1999.
The definitive version is available at http://dx.doi.org/10.1007/BF02467190
Keywords and Phrases
Iron; Accuracy; Capillary Electrophoresis; Chemical Analysis; Iron Blood Level; Metal Binding; Priority Journal; Serum Analysis; Total Iron Binding Capacity
International Standard Serial Number (ISSN)
Article - Journal
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