Pteridine Analysis in Urine by Capillary Electrophoresis using Laser- Induced Fluorescence Detection
Pteridines are a class of compounds excreted in urine, the levels of which are found to elevate significantly in tumor-related diseases. For the first time, we have developed a method, based on high-performance capillary electrophoresis (HPCE) and laser-induced fluorescence (LIF) detection, to monitor the pteridine levels in urine. HPCE provides better separation than high-performance liquid chromatography and the LIF detector enables us to detect minute amounts of pteridines in body fluid. Eight different pteridine derivatives were well separated in 0.1 M Tris-0.1 M borate-2 mM EDTA buffer (pH 8.75) using a 60-cm fused-silica capillary (50-µm i.d., 35-cm effective length), six of which were detected and characterized in urine samples from normal persons and different cancer patients. The detection limits of these pteridines are under 1 x 10-10 M. The levels of neopterin, pterine, xanthopterin, and pterin-6-carboxylic acid were found to be significantly elevated in urine excreted by cancer patents, while the level of isoxanthopterin dropped in these patients. No significant change of biopterin level was found between healthy individuals and cancer patients. This method can be used in clinical laboratories either for cancer monitoring or for precancer screening.
F. Han et al., "Pteridine Analysis in Urine by Capillary Electrophoresis using Laser- Induced Fluorescence Detection," Analytical Chemistry, vol. 71, no. 7, pp. 1265-1269, American Chemical Society (ACS), Apr 1999.
The definitive version is available at http://dx.doi.org/10.1021/ac981218v
Keywords and Phrases
Isoxanthopterin; Neopterin; Pteridine; Pterin Derivative; Xanthopterin; Analytic Method; Article; Capillary Electrophoresis; Chemical Analysis; Controlled Study; Fluorescence; Human; PH; Temperature Dependence; Urinalysis; Electrophoresis, Capillary; Fluorescence; Humans; Lasers; Pteridines
International Standard Serial Number (ISSN)
Article - Journal
© 1999 American Chemical Society (ACS), All rights reserved.