Title

Gold Nanoparticle Based Immunostrip Assay Method for Detection of Protein-A

Abstract

We have successfully developed gold nanoparticle based immunostrip assay to detect protein-A (PA). Rabbit polyclonal antibody IGg (αPA) that has affinity to PA was conjugated to gold nanoparticles (GNPs) and the gold nanoconjugate (αPA-GNP) was used to detect protein-A by simple immunostrip assay method. ELISA experiments were used to confirm the retention of binding affinity of antibody towards protein-A after conjugation with gold nanoparticles.

Department(s)

Chemistry

Sponsor(s)

Science and Engineering Institute; Universiti Putra Malaysia

Keywords and Phrases

Assay methods; Binding affinities; ELISA; Gold nanoparticle; Gold Nanoparticles; Polyclonal antibody; Protein-A; Secondary antibodies; Antibodies; Binding energy; Metal nanoparticles; Gold; αPA; ELISA; Gold nanoparticles (GNP); Immunostrip assay; Protein-A (PA); Secondary antibody (GAR-IgG-HRP)

International Standard Book Number (ISBN)

9783037855102

International Standard Serial Number (ISSN)

16609336

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2012 Trans Tech Publications, All rights reserved.

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