Measurement of Glutathione, Glutathione Disulfide, and Other Thiols in Mammalian Cell and Tissue Homogenates Using High-performance Liquid Chromatography Separation of N-(1-pyrenyl)maleimide Derivatives
Abstract
As glutatione and cellular thiol-related research progresses, the role of these thiols has been extended from acting only in the maintenance of steady-state redox equilibrium, to a proposed involvement in many stressinduced metabolic and bioregulatory functions, including signal transduction and gene expression. 1-3 Glutathione depletion has been identified in various disease processes, including Parkinson's disease and human immunodeficiency virus (HIV) infection. 4,5 Thiols are also known to protect against cytotoxicity associated with exposure to radiation and chemotherapeutic agents. 6'7 Therefore, the evaluation of cellular thiol status may pro-
Recommended Citation
L. A. Ridnour et al., "Measurement of Glutathione, Glutathione Disulfide, and Other Thiols in Mammalian Cell and Tissue Homogenates Using High-performance Liquid Chromatography Separation of N-(1-pyrenyl)maleimide Derivatives," Methods in Enzymology, Elsevier, Jan 1999.
The definitive version is available at https://doi.org/10.1016/S0076-6879(99)99025-0
Department(s)
Chemistry
International Standard Serial Number (ISSN)
0076-6879
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 1999 Elsevier, All rights reserved.
Publication Date
01 Jan 1999
