Title

Measurement of Glutathione, Glutathione Disulfide, and Other Thiols in Mammalian Cell and Tissue Homogenates Using High-performance Liquid Chromatography Separation of N-(1-pyrenyl)maleimide Derivatives

Abstract

As glutatione and cellular thiol-related research progresses, the role of these thiols has been extended from acting only in the maintenance of steady-state redox equilibrium, to a proposed involvement in many stressinduced metabolic and bioregulatory functions, including signal transduction and gene expression. 1-3 Glutathione depletion has been identified in various disease processes, including Parkinson's disease and human immunodeficiency virus (HIV) infection. 4,5 Thiols are also known to protect against cytotoxicity associated with exposure to radiation and chemotherapeutic agents. 6'7 Therefore, the evaluation of cellular thiol status may pro-

Department(s)

Chemistry

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1999 Elsevier, All rights reserved.


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