Correlations between Molecular Numbers and Molecular Masses in an Evanescent Field and their Applications in Probing Molecular Interactions
Complex numbers: The number of molecules detected in the evanescent field during single-molecule fluorescence imaging decreases with increasing molecular weight in free solution (see graphic; FITC = fluorescein isothiocyanate). This technique could be useful for screening undefined molecular interactions at extremely low concentrations without separation.
H. Gai et al., "Correlations between Molecular Numbers and Molecular Masses in an Evanescent Field and their Applications in Probing Molecular Interactions," Angewandte Chemie - International Edition, vol. 44, no. 32, pp. 5107-5110, Wiley-VCH Verlag, Aug 2005.
The definitive version is available at http://dx.doi.org/10.1002/anie.200500461
Keywords and Phrases
Fluorescence; Molecular Weight; Nucleic Acids; Oligomers; Evanescent Fields; Molecule Counting; Oligonucleotides; Single-molecule Studies; Molecular Dynamics
International Standard Serial Number (ISSN)
Article - Journal
© 2005 Wiley-VCH Verlag, All rights reserved.