Title

Effects of Aspartate on Rabbit Muscle Creatine Kinase and the Salt Induced Molten Globule State

Abstract

The aspartate (Asp)-induced unfolding and the salt-induced folding of creatine kinase (CK) have been studied by measuring enzyme activity, fluorescence emission spectra, circular dichroism (CD) spectra, native polyacrylamide gel electrophoresis and ultraviolet difference spectra. the results showed that Asp caused inactivation and unfolding of CK, with no aggregation during CK denaturation. the kinetics of CK unfolding followed a one phase process. at higher concentrations of Asp (>2.5 mM), the CK dimers were partially dissociated. Inactivation occurred before noticeable conformational change during CK denaturation. Asp denatured CK was mostly reactivated and refolded by dilution. KCl induced the molten globule state with compact structure after CK was denatured with 10 mM Asp. These results suggest that the effect of Asp differed from that of other denaturants such as guanidine, HCl or urea during CK unfolding. Asp is a reversible protein denaturant and the molten globule state indicates that intermediates exist during CK folding.

Department(s)

Chemistry

International Standard Serial Number (ISSN)

13572725

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2002 Elsevier, All rights reserved.


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