2',2'-Ligation Demonstrates the Thermal Dependence of DNA-directed Positional Control
An oligodeoxynucleotide (ODN) containing a 2'-aminoalkylmercaptouridine was ligated chemically with another ODN containing a 2'-carboxyalkylmercaptouridine to produce a 2',2'-amide linked conjugate. Templation by a DNA hairpin resulted in ligation yields of >95%. at room temperature, similar yields were obtained for amide ligations between pendent nucleotides located adjacent to each other or separated by three nucleotides. However, at 4 °C, ligation was vastly favored for the case where the pendent amine and carboxylate were attached to adjacent residues (i.e., at the 3'- and 5'-terminus of the two ODNs). No reaction was observed to occur either at 4 °C or at room temperature between pendent nucleotides separated by nine nucleotides. Denaturing gel electrophoresis, MALDI-TOF MS, and full nuclease digestion coupled with LC-MS were used to characterize the reaction products.
Y. Liu et al., "2',2'-Ligation Demonstrates the Thermal Dependence of DNA-directed Positional Control," Tetrahedron, vol. 64, no. 36, pp. 8417-8422, Elsevier, Jan 2008.
The definitive version is available at http://dx.doi.org/10.1016/j.tet.2008.05.046
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