Transition Metal Ion Binding Studies of Carnosine and Histidine: Biologically Relevant Antioxidants
Carnosine and histidine are biologically interesting antioxidants. In order to probe whether they exert their antioxidant effect through metal ion chelation, the Cu(II) ion chelating abilities of these compounds were measured by UV-vis spectroscopy. Both of these compounds showed 1:1 complexations with Cu(II) ions as shown by their Job's plot. The binding constants for histidine and carnosine, as determined by Benesi-Hildebrand method, at pH 7.84 ± 0.18, were found to be 71 and 1.1 M-1, respectively. The unexpectedly lower binding constant values of carnosine show the relatively minor role of the transition metal ion chelation in their antioxidant abilities.
S. Velez et al., "Transition Metal Ion Binding Studies of Carnosine and Histidine: Biologically Relevant Antioxidants," Colloids and Surfaces B: Biointerfaces, vol. 66, no. 2, pp. 291-294, Elsevier, Oct 2008.
The definitive version is available at http://dx.doi.org/10.1016/j.colsurfb.2008.06.012
Keywords and Phrases
Arsenic compounds; Chelation; Chlorine compounds; Copper alloys; Ions; Metal ions; Metals; Transition metal compounds; Transition metals; Ultraviolet spectroscopy; Antioxidant; Antioxidant effect; Benesi-Hildebrand; Binding constants; Carnosine; Cu (II); Histidine; Job's plot; Metal ion binding; Transition-metal ions; UV-VIS spectroscopy; Complexation; chelating agent; copper ion; binding affinity; chelation; complex formation; methodology; pH; priority journal; Binding Sites; Binding, Competitive; Hydrogen-Ion Concentration; Ions; Molecular Structure; Spectrophotometry, Ultraviolet; Surface Properties
Library of Congress Subject Headings
International Standard Serial Number (ISSN)
Article - Journal
© 2008 Elsevier, All rights reserved.