Supercritical Fluid Extraction and Enzyme Immunoassay for Pesticide Detection in Meat Products
Two techniques supercritical fluid extraction (SFE) and enzyme immunoassay (EIA) were integrated into an analytical method for the rapid detection of pesticide residues in meat samples. The pesticides of interest were extracted from meats using supercritical CO2. A pumpless SFE system, which was designed in our laboratory, and commercial equipment were used in SFE experiments. The presence of pesticide residues in the extract was quantitatively determined using the magnetic bead-based EIA kits. Several types of pesticides (alachlor, carbofuran, atrazine, benomyl, and 2,4-D), spiked in the meat samples (bovine liver, ground beef, and lard), were extracted and analyzed. Interferences caused by the coextracted substances from these complex sample matrices required the use of a cleanup step prior to the EIA test. The described techniques are potentially portable and could be used for the rapid screening of meat samples in plant environments.
P. K. Nam and J. W. King, "Supercritical Fluid Extraction and Enzyme Immunoassay for Pesticide Detection in Meat Products," Journal of Agricultural and Food Chemistry, vol. 42, no. 7, pp. 1469-1474, American Chemical Society (ACS), Jul 1994.
The definitive version is available at http://dx.doi.org/10.1021/jf00043a015
Keywords and Phrases
Extraction; Immunoassay; Meats; Pesticide; Supercritical Fluid
International Standard Serial Number (ISSN)
Article - Journal
© 1994 American Chemical Society (ACS), All rights reserved.