Coupling Enzyme Immunoassay with Supercritical Fluid Extraction
The coupling of enzyme immunoassay with supercritical fluid extraction (SFE) is an attractive technique for analysts faced with decreasing the use of hazardous solvents, due to their adverse impact on the environment. This chapter will describe the development of supercritical fluid extraction techniques which can be combined with enzyme immunoassays for the detection of pesticide residues and similar toxicants in food and environmental samples. The use of static versus dynamic SFE will be contrasted with respect to speed of analysis, equipment requirements, and quantitative vs. qualitative analysis. Detection of the presence of pesticides in meat matrices was accomplished using different commercial test kits. Removal of various interferences from the sample extract prior to EIA is necessary to achieve quantitative results, due to the presence of lipid coextractives in the extract. The above techniques have been successfully employed to determine pesticide residue content in meat products and other matrices below their specified tolerance limit set by regulatory agencies.
J. W. King and P. K. Nam, "Coupling Enzyme Immunoassay with Supercritical Fluid Extraction," ACS Symposium Series, American Chemical Society (ACS), Jan 1996.
The definitive version is available at http://dx.doi.org/10.1021/bk-1996-0621.ch034
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© 1996 American Chemical Society (ACS), All rights reserved.