Title

Correlations between Molecular Numbers and Molecular Masses in an Evanescent Field and their Applications in Probing Molecular Interactions

Abstract

Complex numbers: The number of molecules detected in the evanescent field during single-molecule fluorescence imaging decreases with increasing molecular weight in free solution (see graphic; FITC = fluorescein isothiocyanate). This technique could be useful for screening undefined molecular interactions at extremely low concentrations without separation.

Department(s)

Chemistry

Keywords and Phrases

Evanescent Field; Fluorescence; Molecule Counting; Oligonucleotides; Single-molecule Studies

International Standard Serial Number (ISSN)

1433-7851

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2005 Wiley-VCH Verlag Berlin, All rights reserved.


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