Structure-Based Kinetic Models of Modular Signaling Protein Function: Focus on Shp2

Abstract

We present here a computational, rule-based model to study the function of the SH2 domain-containing protein tyrosine phosphatase, Shp2, in intracellular signal transduction. The two SH2 domains of Shp2 differentially regulate the enzymatic activity by a well-characterized mechanism, but they also affect the targeting of Shp2 to signaling receptors in cells. Our kinetic model integrates these potentially competing effects by considering the intra- and intermolecular interactions of the Shp2 SH2 domains and catalytic site as well as the effect of Shp2 phosphorylation. Even for the isolated Shp2/receptor system, which may seem simple by certain standards, we find that the network of possible binding and phosphorylation states is composed of over 1000 members. To our knowledge, this is the first kinetic model to fully consider the modular, multifunctional structure of a signaling protein, and the computational approach should be generally applicable to other complex intermolecular interactions.

Department(s)

Chemical and Biochemical Engineering

Keywords and Phrases

Protein Tyrosine Phosphatase SHP 2, Article; Enzyme Activity; Kinetics; Mathematical Model; Molecular Dynamics; Protein Binding; Protein Interaction; Protein Phosphorylation; Protein Structure; Signal Transduction

International Standard Serial Number (ISSN)

0006-3495

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2007 Biophysical Society, All rights reserved.

Publication Date

01 Apr 2007

PubMed ID

17208977

Link to Full Text

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