Impact of Nanopore Morphology on Cell Viability on Mesoporous Polymer and Carbon Surfaces

Abstract

Topography at the nanoscale can lead to dramatic changes in the adhesion of cells to surfaces and their subsequent viability. For biological applications, including tissue engineering and cell-based sensing, the large internal surface area of ordered mesoporous carbons provides an opportunity for enhanced sensitivity and performance, but the mesostructure also affects the topography of the material. In this work, we probe the viability and adhesion of osteoblasts on ordered mesoporous materials with different morphologies and matrix chemistries. FDU-15 (hexagonal) and FDU-16 (cubic) films were processed at either 350⁰ (polymeric) or 800⁰ (carbon) to provide these different materials. For the films processed at 350⁰, the cell adhesion was markedly improved on the mesoporous films in comparison to a dense film analog, consistent with many reports in the literature that nanostructuring of surfaces improves the viability and adhesion of osteoblasts. Conversely, osteoblast adhesion was reduced on the carbonized surfaces processed at 800 ° when ordered mesopores were introduced, particularly for the cubic mesostructure (FDU-16). We attribute the decrease in cell adhesion to the propensity of the ordered mesoporous carbon films to sorb organics from aqueous solution, which could lead to removal of adhesionpromoting compounds at the film surface. These results suggest that cell viability on mesoporous polymer and carbon films can be controlled through simple changes in the pyrolysis temperature.

Department(s)

Chemical and Biochemical Engineering

Keywords and Phrases

Carbon; Cell adhesion; Mesoporous films; Nanostructures; Osteoblasts

International Standard Serial Number (ISSN)

1742-7061

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2010 Elsevier BV, All rights reserved.

Publication Date

01 Aug 2010

PubMed ID

20144750

Link to Full Text

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