Discovery of Cationic Polymers for Non-Viral Gene Delivery Using Combinatorial Approaches
Gene therapy is an attractive treatment option for diseases of genetic origin, including several cancers and cardiovascular diseases. While viruses are effective vectors for delivering exogenous genes to cells, concerns related to insertional mutagenesis, immunogenicity, lack of tropism, decay and high production costs necessitate the discovery of non-viral methods. Significant efforts have been focused on cationic polymers as non-viral alternatives for gene delivery. Recent studies have employed combinatorial syntheses and parallel screening methods for enhancing the efficacy of gene delivery, biocompatibility of the delivery vehicle, and overcoming cellular level barriers as they relate to polymermediated transgene uptake, transport, transcription, and expression. This review summarizes and discusses recent advances in combinatorial syntheses and parallel screening of cationic polymer libraries for the discovery of efficient and safe gene delivery systems.
S. Barua et al., "Discovery of Cationic Polymers for Non-Viral Gene Delivery Using Combinatorial Approaches," Combinatorial Chemistry and High Throughput Screening, vol. 14, no. 10, pp. 908-924, Bentham Science Publishers, Dec 2011.
The definitive version is available at https://doi.org/10.2174/138620711797537076
Chemical and Biochemical Engineering
Keywords and Phrases
Cytotoxicity; Gene delivery; Parallel screening; Polymer library; Transfection; Transgene expression
International Standard Serial Number (ISSN)
Article - Journal
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