Cancer-Cell-Phenotype-Dependent Differential Intracellular Trafficking of Unconjugated Quantum Dots
A diverse array of nanoparticles, including quantum dots (QDs), metals, polymers, liposomes, and dendrίmers, are being investigated as therapeutics and imaging agents in cancer diseases. However, the role of the cancer-cell phenotype on the uptake and intracellular fate of nanoparticles in cancer cells remains poorly understood. Reported here is that differences in cancer- cell phenolypes can lead to significant differences in intracellular sorting, trafficking, and localization of nanoparticles. Unconjugated anionic QDs demonstrate dramatically different intracellular profiles in three closely related human-prostate-cancer cells used in the investigation: PC3, PC3-flu, and PC3-PSMA. QDs demonstrate punctated intracellular localization throughout the cytoplasm in PC3 cells. In contrast, the nanoparticles localize mainly at a single juxtanuclear location (" dot-of-dots") inside the perinuclear recycling compartment in PC3-PSMA cells, where they co- localize with transferrin and the prostate-specific membrane antigen. The results indicate that nanoparticle sorting and transport is influenced by changes in cancer-cell phenotype and can have significant implications in the design and engineering of nanoscale drug delivery and imaging systems for advanced tumors
S. Barua and K. Rege, "Cancer-Cell-Phenotype-Dependent Differential Intracellular Trafficking of Unconjugated Quantum Dots," Small, vol. 5, no. 3, pp. 370-376, John Wiley & Sons Inc., Feb 2009.
The definitive version is available at https://doi.org/10.1002/smll.200800972
Chemical and Biochemical Engineering
Keywords and Phrases
Intracellular transport; Microtubules; Nanoparticle trafficking; Perinuclear recycling com-partment; Quantum dots
International Standard Serial Number (ISSN)
Article - Journal
© 2009 John Wiley & Sons Inc., All rights reserved.