Surface Enrichment of Proteins at Quartz / Water Interfaces: A Neutron Reflectivity Study
Neutron reflectivity (NR) was used to study the adsorption of human serum albumin and human fibrinogen on quartz. The proteins were individually and sequentially adsorbed from heavy water and heavy water/methanol mixtures at pH 4 and 7.0. The technique allows for the subnanometer resolution of the adsorbed layer thickness and gross morphology. under the conditions of our measurements we found that fibrinogen formed a distinct layer that we interpret as a mat of the protein three layers thick whereas albumin formed only diffuse layers. The adsorption pattern of the two proteins changed radically when one protein was adsorbed on top of the other (previously adsorbed). In general our measurements indicate that the adsorbed protein layers on quartz are rather loosely bound and that these layers, incorporating as much as 80% water, extend further into the bulk fluid than might have been expected.
D. Forciniti and W. Hamilton, "Surface Enrichment of Proteins at Quartz / Water Interfaces: A Neutron Reflectivity Study," Journal of Colloid and Interface Science, Elsevier, May 2005.
The definitive version is available at http://dx.doi.org/10.1016/j.jcis.2004.11.060
Chemical and Biochemical Engineering
National Science Foundation (U.S.)
Keywords and Phrases
Albumin; Fibrinogen; Neutron Reflectivity; Protein Adsorption; Sequential Adsorption
Article - Journal
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