Abstract

Bioprinting research is focused on utilizing growth factors and multiple cell types to create clinically relevant three-dimensional (3D) tissue models using hydrogels. Rheological and biological challenges are two main factors that limit the creation of extrudable bioactive hydrogels. In this study, we investigate incorporation of fast dissolving and bioactive borate glass in different weight to volume percentages (0.075 to 0.6%) to alginate-gelatin (1:1) hydrogel to improve rheological properties and enable bioprinting with bioactive glass. The addition of glass improved the stiffness of the hydrogel. Human adipose-derived mesenchymal stem cells (ASCs) were uniformly mixed in this bioink at 1 x 106 cells/mL concentration, and spheroid specimens were cultured in both static and dynamic culture conditions. Grid-shaped scaffolds measuring ~18 x 18 x 1 mm3 were fabricated with the viable glass concentrations, and ASC viability was evaluated using Live/Dead assay. Despite immediate toxicity, an increased viability after 7 days with 0.15 w/v % or less borate glass content demonstrated the potential in utilizing highly resorbable calcium-releasing biomaterials such as bioactive glasses to modify hydrogels suitable for bioprinting cellularized 3D structures.

Department(s)

Biological Sciences

Second Department

Materials Science and Engineering

Third Department

Mechanical and Aerospace Engineering

Publication Status

Open Access

Comments

Intelligent Systems Center, Grant None

Keywords and Phrases

Adipose stem cells; Alginate; Bioactive glass; Bioink; Ceramics; Gelatin

International Standard Serial Number (ISSN)

2424-8002

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2024 The Authors, All rights reserved.

Publication Date

01 Jan 2024

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